CLEC4C
(C-Type Lectin Domain Family 4, Member C (CLEC4C))
Protein-Typ
Recombinant
Proteineigenschaft
AA 46-213
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses CLEC4C Protein ist gelabelt mit His tag.
Verwendungszweck
Human BDCA-2 Protein
Sequenz
Phe46-Ile213
Produktmerkmale
Recombinant Human BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe46-Ile213.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human BDCA-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
CLEC4C
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CLEC4C
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 20 mM Tris, 300 mM NaCl, 200 mM L-arginine ( pH 8.5).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
CLEC4C
(C-Type Lectin Domain Family 4, Member C (CLEC4C))
BDCA2 Protein, CD303 Protein, CLECSF11 Protein, CLECSF7 Protein, DLEC Protein, HECL Protein, PRO34150 Protein, Clec4c Protein, C-type lectin domain family 4 member C Protein, CLEC4C Protein, LOC100733227 Protein
Hintergrund
BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
Molekulargewicht
20.93 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.