TRAIL (TNFSF10)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
Protein-Typ
Recombinant
Proteineigenschaft
AA 114-281
Spezies
Human
Quelle
Escherichia coli (E. coli)
Verwendungszweck
Human TRAIL/TNFRSF10 Protein
Sequenz
Val114-Gly281
Produktmerkmale
Recombinant Human TRAIL/TNFRSF10 Protein is expressed from E.coli without tag.It contains Val114-Gly281.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TRAIL at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 51.6ng/ml determined by ELISA. The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
TNFSF10
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
Crystallography grade
TNFSF10
Spezies: Maus
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 50 mM Tris, 300 mM NaCl ( pH 7.2).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
TRAIL (TNFSF10)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents.