RANKL Protein (AA 63-244)

Produktnummer ABIN7275516

Produktdetails

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Protein-Typ: Recombinant

Proteineigenschaft: AA 63-244

Spezies: Human

Quelle: HEK-293 Cells

Verwendungszweck: Human RANKL/TNFSF11/CD254 Protein

Sequenz: Gly63-Asp244

Produktmerkmale: Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 without tag.It contains Gly63-Asp244.

Reinheit: > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Andere Bezeichnung: RANKL (TNFSF11 Produkte)

Synonyme: CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, TNFSF11 Protein, Ly109l Protein, OPG Protein, Trance Protein, cd254 Protein, odf Protein, opgl Protein, optb2 Protein, rankl Protein, sodf Protein, tnfsf11 Protein, trance Protein, TNF superfamily member 11 Protein, tumor necrosis factor (ligand) superfamily, member 11 Protein, TNF receptor superfamily member 11b Protein, TNF superfamily member 11 L homeolog Protein, TNFSF11 Protein, Tnfsf11 Protein, TNFRSF11B Protein, tnfsf11.L Protein

Hintergrund: Receptor activator of nuclear factor x03BA,B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

Molekulargewicht: 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu

Pathways: NF-kappaB Signalweg