PCSK9
(Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
Protein-Typ
Recombinant
Proteineigenschaft
AA 31-692
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses PCSK9 Protein ist gelabelt mit His tag.
Verwendungszweck
Human PCSK9 Protein
Sequenz
Gln31-Gln692
Produktmerkmale
Recombinant Human PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln31-Gln692.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human PCSK9, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. The affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
PCSK9
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Crystallography grade
PCSK9
Spezies: Maus
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 20 mM PB, 300 mM NaCl, 10 % Glycerol ( pH 7.4).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
PCSK9
(Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
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Hintergrund
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1.The first two PCSK9 inhibitors, alirocumab and evolocumab, were approved as once every two week injections, by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.
Molekulargewicht
Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result.