TNFSF4 Protein (Trimer) (His-DYKDDDDK Tag)
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- Target Alle TNFSF4 Proteine anzeigen
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- Trimer
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses TNFSF4 Protein ist gelabelt mit His-DYKDDDDK Tag.
- Verwendungszweck
- Human OX40 Ligand/TNFSF4 Trimer Protein
- Sequenz
- Gln51-Leu183
- Produktmerkmale
- Recombinant Human OX40 Ligand/TNFSF4 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gln51-Leu183.
- Reinheit
- > 90 % as determined by Tris-Bis PAGE
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human OX40 Ligand Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Andere Bezeichnung
- OX40 Ligand (TNFSF4 Produkte)
- Synonyme
- TNFSF4 Protein, OX40L Protein, TNFRSF4 Protein, CD134L Protein, CD252 Protein, GP34 Protein, OX-40L Protein, OX4OL Protein, TXGP1 Protein, Ath-1 Protein, Ath1 Protein, Ox40l Protein, Txgp1l Protein, gp34 Protein, TNF superfamily member 4 Protein, tumor necrosis factor (ligand) superfamily, member 4 Protein, TNFSF4 Protein, Tnfsf4 Protein
- Hintergrund
- Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
- Molekulargewicht
- 49.7 kDa. Due to glycosylation, the protein migrates to 65-140 kDa based on Tris-Bis PAGE result.
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints
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