PVRL2 Protein (AA 32-360) (His-Avi Tag)

Produktnummer ABIN7275309

Produktdetails

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 32-360

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PVRL2 Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human Nectin-2/CD112 Protein

Sequenz: Gln32-Leu360

Produktmerkmale: Recombinant Human Nectin-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln32-Leu360.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Nectin-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.80μg/ml determined by ELISA. The affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Andere Bezeichnung: Nectin-2 (PVRL2 Produkte)

Synonyme: prr2 Protein, AI325026 Protein, AI987993 Protein, Cd112 Protein, MPH Protein, Pvr Protein, Pvs Protein, nectin-2 Protein, CD112 Protein, HVEB Protein, PRR2 Protein, PVRR2 Protein, nectin cell adhesion molecule 2 Protein, Nectin2 Protein, NECTIN2 Protein

Hintergrund: Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.

Molekulargewicht: 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization