Kallikrein 5 Protein (KLK5) (AA 23-293) (His tag)

Produktnummer ABIN7275095

Produktdetails

Target: Kallikrein 5 (KLK5)

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-293

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Kallikrein 5 Protein ist gelabelt mit His tag.

Verwendungszweck: Human Kallikrein 5/KLK5 Protein

Sequenz: Val23-Ser293

Produktmerkmale: Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val23-Ser293.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Kallikrein 5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCl ( pH 5.5).

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl ( pH 5.5). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Kallikrein 5 (KLK5)

Andere Bezeichnung: Kallikrein 5 (KLK5 Produkte)

Synonyme: KLK-L2 Protein, KLKL2 Protein, SCTE Protein, 1110030O19Rik Protein, KAL Protein, KALA Protein, Klk1 Protein, Klk5 Protein, RATKALA Protein, kallikrein related peptidase 5 Protein, kallikrein related-peptidase 5 Protein, kallikrein 5-like Protein, KLK5 Protein, Klk5 Protein, Klk5l Protein

Hintergrund: The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.

Molekulargewicht: 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Tris-Bis PAGE result.

UniProt: Q9Y337

Pathways: Komplementsystem, Regulation of G-Protein Coupled Receptor Protein Signaling