Dieses IL-4 Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human IL-4 Protein
Sequenz
His25-Ser153
Produktmerkmale
Recombinant Biotinylated Human IL-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His25-Ser153.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-4 R alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
IL4
Spezies: Human
Wirt: Wheat germ
Recombinant
WB, ELISA, AP, AA
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.
Molekulargewicht
17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.