IL-31 Protein (AA 24-164) (His tag)

Produktnummer ABIN7274942

Produktdetails

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Protein-Typ: Recombinant

Proteineigenschaft: AA 24-164

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL-31 Protein ist gelabelt mit His tag.

Verwendungszweck: Human IL-31 Protein

Sequenz: Ser24-Thr164

Produktmerkmale: Recombinant Human IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser24-Thr164.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-31, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.2μg/ml determined by ELISA. The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Andere Bezeichnung: IL-31 (IL31 Produkte)

Synonyme: IL-31 Protein, 1700013B14Rik Protein, IL31 Protein, interleukin 31 Protein, IL31 Protein, Il31 Protein

Hintergrund: Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.

Molekulargewicht: 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.