IL-31 Protein (AA 27-163) (His tag)

Produktnummer ABIN7274936

Recombinant IL-31-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für IL-31 Protein (AA 27-163) (His tag) (ABIN7274936)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE

Produktdetails

Proteineigenschaft: AA 27-163

Aufreinigungstag / Konjugat: Dieses IL-31 Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus IL-31 Protein

Sequenz: Leu27-Thr163

Produktmerkmale: Recombinant Cynomolgus IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu27-Thr163.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus IL-31, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-31 RA, His Tag with an affinity constant of 6.83 nM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus IL-31, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 0.59 ug/ml determined by ELISA (QC Test).,Cynomolgus IL-31 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Andere Bezeichnung: IL-31

Hintergrund: Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.

Molekulargewicht: 16.5 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.

UniProt: G7PJ78