FcRn Protein (AA 24-297) (His tag)
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- Target Alle FcRn Proteine anzeigen
- FcRn (IgG receptor FcRn (FcRn))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 24-297
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses FcRn Protein ist gelabelt mit His tag.
- Verwendungszweck
- Human FcRn Protein
- Sequenz
- Ala24-Ser297 (FCGRT) & Ile21-Met119 (B2M)
- Spezifität
- Uni-Prot: P55899-1 (FCGRT), P61769-1 (B2M)
- Produktmerkmale
- Recombinant Human FcRn Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala24-Ser297(FCGRT) & Ile21-Met119(B2M).
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FcRn, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA. The affinity constant of 0.283 μM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product FcRn Protein
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- FcRn (IgG receptor FcRn (FcRn))
- Andere Bezeichnung
- FcRn (FcRn Produkte)
- Synonyme
- FCRN Protein, alpha-chain Protein, FcRn Protein, Fc fragment of IgG receptor and transporter Protein, Fc receptor, IgG, alpha chain transporter Protein, FCGRT Protein, Fcgrt Protein
- Hintergrund
- The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
- Molekulargewicht
- 31.5 kDa (FCGRT) & 11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT) & 13-14 kDa (B2M) based on Tris-Bis PAGE result.
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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