FCGR1 Protein (AA 25-297) (His tag)

Produktnummer ABIN7274674

Produktdetails

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-297

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FCGR1 Protein ist gelabelt mit His tag.

Verwendungszweck: Mouse Fc gamma RI/CD64 Protein

Sequenz: Glu25-Pro297

Produktmerkmale: Recombinant Mouse Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Pro297.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Andere Bezeichnung: Fc gamma RI (FCGR1 Produkte)

Synonyme: CD64 Protein, CD64A Protein, FCRI Protein, IGFR1 Protein, AI323638 Protein, AV092959 Protein, FcgammaRI Protein, IGGHAFC Protein, FCGR1A Protein, Fcgr1 Protein, Fcgr1b Protein, Fc fragment of IgG receptor Ia Protein, Fc receptor, IgG, high affinity I Protein, FCGR1A Protein, Fcgr1 Protein, Fcgr1a Protein

Hintergrund: The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

Molekulargewicht: 31.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process