FCGR1 Protein (His tag)

Produktnummer ABIN7195718

Produktdetails

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FCGR1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse CD64/FCGR1 Protein (His Tag)(Active)

Sequenz: Met 1-Pro 297

Produktmerkmale: A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) precursor was fused with a polyhistidine tag at the C-terminus.

Reinheit: > 97 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized mouse CD64-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.07-0.17 μg/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Andere Bezeichnung: CD64/FCGR1 (FCGR1 Produkte)

Synonyme: CD64 Protein, CD64A Protein, FCRI Protein, IGFR1 Protein, AI323638 Protein, AV092959 Protein, FcgammaRI Protein, IGGHAFC Protein, FCGR1A Protein, Fcgr1 Protein, Fcgr1b Protein, Fc fragment of IgG receptor Ia Protein, Fc receptor, IgG, high affinity I Protein, FCGR1A Protein, Fcgr1 Protein, Fcgr1a Protein

Hintergrund:

Background: High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Synonym: High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; CD64;FcgammaRI;IGGHAFC

Molekulargewicht: 32 kDa

NCBI Accession: NP_034316

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process