FLT3 Protein (AA 28-541) (Fc Tag)

Produktnummer ABIN7274655

Produktdetails

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 28-541

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FLT3 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Cynomolgus FLT3/Flk-2 Protein

Sequenz: Gln28-Ser541

Produktmerkmale: Recombinant Cynomolgus FLT3/Flk-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln28-Ser541.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FLT3 Ligand, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Cynomolgus FLT3, hFc Tag with the EC50 of 18ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Andere Bezeichnung: FLT3 (FLT3 Produkte)

Synonyme: FLT3 Protein, B230315G04 Protein, CD135 Protein, Flk-2 Protein, Flk2 Protein, Flt-3 Protein, Ly72 Protein, wmfl Protein, FLK-2 Protein, FLK2 Protein, STK1 Protein, fms related tyrosine kinase 3 Protein, FMS-like tyrosine kinase 3 Protein, fms-related tyrosine kinase 3 Protein, FLT3 Protein, Flt3 Protein

Hintergrund: The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.

Molekulargewicht: 84.58 kDa. Due to glycosylation, the protein migrates to 160-180 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5VQ48

Pathways: RTK Signalweg