FAP Protein (His tag)

Produktnummer ABIN7274583

Produktdetails

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FAP Protein ist gelabelt mit His tag.

Sequenz: Leu26-Asp760

Reinheit: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FAP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris, 250 mM NaCl ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Andere Bezeichnung: FAP (FAP Produkte)

Synonyme: fap-A Protein, gene 13 Protein, FAP Protein, fapa Protein, dppiv Protein, seprase Protein, DPPIV Protein, FAPA Protein, fibroblast activation protein Protein, fibroblast activation protein alpha L homeolog Protein, fibroblast activation protein alpha Protein, fibroblast activation protein, alpha Protein, prolyl endopeptidase FAP Protein, Fap Protein, fap.L Protein, FAP Protein, fap Protein, LOC100547273 Protein

Hintergrund: FAP, FAPalpha, SIMP, Seprase, APCE, DPPIV, DPPIVA, FAPA,Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

Molekulargewicht: 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.

Pathways: Tube Formation