CLEC12A
(C-Type Lectin Domain Family 12, Member A (CLEC12A))
Protein-Typ
Recombinant
Proteineigenschaft
AA 32-232
Spezies
Cynomolgus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses CLEC12A Protein ist gelabelt mit His tag.
Verwendungszweck
Cynomolgus CLEC12A/MICL/CLL-1 Protein
Sequenz
His32-Ala232
Produktmerkmale
Recombinant Cynomolgus CLEC12A/MICL/CLL-1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains His32-Ala232.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Anti-CLEC12A Antibody captured on CM5 Chip via Protein A can bind Cynomolgus CLEC12A, His Tag with an affinity constant of 1.28 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
CLEC12A
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
CLEC12A
(C-Type Lectin Domain Family 12, Member A (CLEC12A))
MGC179455 Protein, CLL-1 Protein, CLL1 Protein, DCAL-2 Protein, MICL Protein, D230024O04 Protein, KLRL1 Protein, Micl Protein, C-type lectin domain family 12 member A Protein, C-type lectin domain family 12, member a Protein, C-type lectin domain family 12, member A Protein, CLEC12A Protein, Clec12a Protein
Hintergrund
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.
Molekulargewicht
24.6 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.