CD3E & CD3G (AA 22-117) protein (Fc Tag)
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- Target
- CD3E & CD3G
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 22-117
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Spezies
- Cynomolgus
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Fc Tag
- Verwendungszweck
- Cynomolgus CD3E & CD3G/CD3 epsilon & CD3 gamma Protein
- Sequenz
- Gln22-Asp117 (CD3E) & Gln23-Thr113 (CD3G)
- Spezifität
- Uni-Prot: Q95LI5.2 (CD3E), Q95LI7 (CD3G)
- Produktmerkmale
- Recombinant Cynomolgus CD3E & CD3G/CD3 epsilon & CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G).
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA. See testing image for detail.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- CD3E & CD3G
- Andere Bezeichnung
- CD3E&CD3G
- Hintergrund
- T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
- Molekulargewicht
- 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.
- UniProt
- Q95LI5
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