CD3E & CD3G (AA 22-117) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)

Produktnummer ABIN6973006

Produktdetails

Target: CD3E & CD3G

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 22-117

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: His tag,AVI tag,DYKDDDDK Tag,Biotin

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: MALS verified

Kommentare:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Antigendetails

Target: CD3E & CD3G

Sub Type: Cocktail

Hintergrund: T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Molekulargewicht: 17.7 kDa (CD3E) & 15.4 kDa (CD3G)