VTCN1 Protein (His-Avi Tag,Biotin)

Produktnummer ABIN7273953

Produktdetails

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses VTCN1 Protein ist gelabelt mit His-Avi Tag,Biotin.

Sequenz: Phe29-Ala258

Reinheit: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-B7-H4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human B7-H4, His Tag with the EC50 of 24.3ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Andere Bezeichnung: B7-H4 (VTCN1 Produkte)

Synonyme: B7h4 Protein, B7s1 Protein, B7x Protein, BC032925 Protein, B7-H4 Protein, B7H4 Protein, B7S1 Protein, B7X Protein, B7h.5 Protein, PRO1291 Protein, VCTN1 Protein, RGD1311204 Protein, V set domain containing T cell activation inhibitor 1 L homeolog Protein, V-set domain containing T cell activation inhibitor 1 Protein, V-set domain containing T-cell activation inhibitor 1 Protein, V set domain containing T cell activation inhibitor 1 Protein, vtcn1.L Protein, VTCN1 Protein, vtcn1 Protein, Vtcn1 Protein

Hintergrund: B7H4, B7-H4, B7S1, B7h.5, B7x, FLJ22418, VTCN1, PRO1291,B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.

Molekulargewicht: 28.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.