TPBG Protein (AA 32-355) (His-Avi Tag,Biotin)
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- Target Alle TPBG Proteine anzeigen
- TPBG (Trophoblast Glycoprotein (TPBG))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 32-355
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses TPBG Protein ist gelabelt mit His-Avi Tag,Biotin.
- Verwendungszweck
- Biotinylated Human TPBG/5T4 Protein
- Sequenz
- Ser32-Ser355
- Produktmerkmale
- Recombinant Biotinylated Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser32-Ser355.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-TPBG Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 84.3ng/ml determined by ELISA. See testing image for detail.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- TPBG (Trophoblast Glycoprotein (TPBG))
- Andere Bezeichnung
- TPBG (TPBG Produkte)
- Synonyme
- 5T4 Protein, 5T4AG Protein, M6P1 Protein, AW495680 Protein, TPBG Protein, tpbg Protein, trophoblast glycoprotein Protein, trophoblast glycoprotein b Protein, TPBG Protein, Tpbg Protein, tpbgb Protein, tpbg Protein
- Hintergrund
- The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
- Molekulargewicht
- 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
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