TPBG Protein (AA 35-355) (His tag)

Produktnummer ABIN7273783

Produktdetails

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Protein-Typ: Recombinant

Proteineigenschaft: AA 35-355

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TPBG Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus TPBG/5T4 Protein

Sequenz: Thr35-Ser355

Produktmerkmale: Recombinant Cynomolgus TPBG/5T4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr35-Ser355.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus TPBG, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Andere Bezeichnung: TPBG (TPBG Produkte)

Synonyme: 5T4 Protein, 5T4AG Protein, M6P1 Protein, AW495680 Protein, TPBG Protein, tpbg Protein, trophoblast glycoprotein Protein, trophoblast glycoprotein b Protein, TPBG Protein, Tpbg Protein, tpbgb Protein, tpbg Protein

Hintergrund: The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.

Molekulargewicht: 35.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

UniProt: Q4R8Y9