2B4 Protein (AA 22-221) (His-Avi Tag,Biotin)

Produktnummer ABIN7273782

Produktdetails

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-221

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses 2B4 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human 2B4/CD244/SLAMF4 Protein

Sequenz: Cys22-Arg221

Produktmerkmale: Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys22-Arg221.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human 2B4, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD48, hFc Tag with the Ec50 of 7.2ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Andere Bezeichnung: 2B4 (CD244 Produkte)

Synonyme: CD244 Protein, 2B4 Protein, NAIL Protein, NKR2B4 Protein, Nmrk Protein, SLAMF4 Protein, C9.1 Protein, F730046O15Rik Protein, Ly90 Protein, CD244 molecule Protein, CD244 natural killer cell receptor 2B4 Protein, CD244 Protein, Cd244 Protein

Hintergrund: 2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.

Molekulargewicht: 24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Tris-Bis PAGE result.