SARS-CoV-2 Spike S2 Protein (E780Q) (His tag,ECD)

Produktnummer ABIN7197829

Produktdetails

Target: SARS-CoV-2 Spike S2

Protein-Typ: Recombinant

Proteineigenschaft: E780Q

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike S2 Protein ist gelabelt mit His tag,ECD.

Verwendungszweck: Recombinant SARS-CoV-2 Spike S2 (E780Q)(ECD, His Tag)

Sequenz: Ser686-Pro1213

Spezifität: E780Q

Produktmerkmale: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S2 (YP_009724390.1, with mutation E780Q) (Ser686-Pro1213) was expressed with the Fc region of human IgG1 at the C-terminus.

Reinheit: > 90 % as determined by SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg protein as determined by the LAL method.

Biological Activity Comment: Testing in progress

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: SARS-CoV-2 Spike S2

Abstract: SARS-CoV-2 Spike S2 Produkte

Synonyme: E2 Protein, Surface Glycoprotein Protein, S Protein

Substanzklasse: Viral Protein

Hintergrund: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Molekulargewicht: 84.7 kDa

NCBI Accession: YP_009724390