CD45 Protein (Fc Tag)

Produktnummer ABIN7320178

Produktdetails

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD45 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Recombinant Mouse CD45/PTPRC Protein (Fc Tag)(Active)

Sequenz: Met1-Lys425

Produktmerkmale: A DNA sequence encoding the mouse PTPRC (NP_035340.3) (Met1-Lys425) was expressed with the Fc region of human IgG1 at the C-terminus.

Reinheit: > 85 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D-His at 10 μg/ml (100 μl/well) can bind mouse PTPRC-Fc with a linear range of 0.625-5 μg/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Andere Bezeichnung: CD45/PTPRC (PTPRC Produkte)

Synonyme: PTPRC Protein, si:dkeyp-47c5.1 Protein, B220 Protein, CD45 Protein, CD45R Protein, GP180 Protein, L-CA Protein, LCA Protein, LY5 Protein, T200 Protein, Cd45 Protein, Ly-5 Protein, Lyt-4 Protein, loc Protein, Lca Protein, RT7 Protein, cd45 Protein, protein tyrosine phosphatase, receptor type C Protein, protein tyrosine phosphatase, receptor type, C Protein, receptor-type tyrosine-protein phosphatase C Protein, PTPRC Protein, ptprc Protein, LOC100074271 Protein, Ptprc Protein

Hintergrund:

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexs or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

Synonym: B220,Cd45,CD45R,L-CA,loc,Ly-5,Lyt-4,T200

Molekulargewicht: 71.7 kDa

NCBI Accession: NP_035340

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling