MDH1 Protein (His tag)

Produktnummer ABIN7318759

Produktdetails

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MDH1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human MDH1 Protein (His Tag)

Sequenz: Ser2-Ala334

Produktmerkmale: Recombinant Human Malate Dehydrogenase, Cytoplasmic is produced by our E.coli expression system and the target gene encoding Ser2-Ala334 is expressed with a 6His tag at the C-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Andere Bezeichnung: MDH1 (MDH1 Produkte)

Synonyme: MDH-s Protein, MDHA Protein, MGC:1375 Protein, MOR2 Protein, ODS1 Protein, B230377B03Rik Protein, D17921 Protein, Mor-2 Protein, Mor2 Protein, MDL1 Protein, Mdhl Protein, MDH Protein, mdh-s Protein, mdha Protein, mor2 Protein, MDH-1 Protein, Mdh Protein, Mdh-1 Protein, Mdh2 Protein, MdhD Protein, cMdh Protein, sMdh Protein, malate dehydrogenase 1 Protein, malic enzyme 2 Protein, malate dehydrogenase 1, NAD (soluble) Protein, malate dehydrogenase 1 S homeolog Protein, malate dehydrogenase, cytoplasmic Protein, Malate dehydrogenase 1 Protein, MDH1 Protein, ME2 Protein, Mdh1 Protein, mdh1.S Protein, LOC100280767 Protein

Hintergrund:

Background: Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.

Synonym: Malate Dehydrogenase Cytoplasmic, Cytosolic Malate Dehydrogenase, Diiodophenylpyruvate Reductase, MDH1, MDHA

Molekulargewicht: 37.5 kDa

UniProt: P40925