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MDH1 Protein (AA 2-334) (His tag)

MDH1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Produktnummer ABIN1097125
  • Target Alle MDH1 Proteine anzeigen
    MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-334
    Spezies
    • 10
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 10
    • 8
    • 2
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses MDH1 Protein ist gelabelt mit His tag.
    Verwendungszweck
    Recombinant Human Malate Dehydrogenase, Cytoplasmic/MDH1 (C-6His)
    Sequenz
    SEPIRVLVTG AAGQIAYSLL YSIGNGSVFG KDQPIILVLL DITPMMGVLD GVLMELQDCA LPLLKDVIAT DKEDVAFKDL DVAILVGSMP RREGMERKDL LKANVKIFKS QGAALDKYAK KSVKVIVVGN PANTNCLTAS KSAPSIPKEN FSCLTRLDHN RAKAQIALKL GVTANDVKNV IIWGNHSSTQ YPDVNHAKVK LQGKEVGVYE ALKDDSWLKG EFVTTVQQRG AAVIKARKLS SAMSAAKAIC DHVRDIWFGT PEGEFVSMGV ISDGNSYGVP DDLLYSFPVV IKNKTWKFVE GLPINDFSRE KMDLTAKELT EEKESAFEFL SSALEHHHHH H
    Produktmerkmale
    Recombinant Human Malate Dehydrogenase, Cytoplasmic/MDH1 is produced by our E. coli expression system. The target protein is expressed with sequence (Ser2-Ala334) of Human MDH1 fused with a 6His tag at the C-terminus.
    Reinheit
    > 95 % as determined by reducing SDS-PAGE.
    Sterilität
    0.2 μm filtered
    Endotoxin-Niveau
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
    Top Product
    Discover our top product MDH1 Protein
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
    Handhabung
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.
    Haltbarkeit
    6 months
  • Target
    MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
    Andere Bezeichnung
    MDH1 (MDH1 Produkte)
    Synonyme
    MDH-s Protein, MDHA Protein, MGC:1375 Protein, MOR2 Protein, ODS1 Protein, B230377B03Rik Protein, D17921 Protein, Mor-2 Protein, Mor2 Protein, MDL1 Protein, Mdhl Protein, MDH Protein, mdh-s Protein, mdha Protein, mor2 Protein, MDH-1 Protein, Mdh Protein, Mdh-1 Protein, Mdh2 Protein, MdhD Protein, cMdh Protein, sMdh Protein, malate dehydrogenase 1 Protein, malic enzyme 2 Protein, malate dehydrogenase 1, NAD (soluble) Protein, malate dehydrogenase 1 S homeolog Protein, malate dehydrogenase, cytoplasmic Protein, Malate dehydrogenase 1 Protein, MDH1 Protein, ME2 Protein, Mdh1 Protein, mdh1.S Protein, LOC100280767 Protein
    Sub Type
    Fusionprotein
    Hintergrund
    Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.
    Alternative Names: Malate Dehydrogenase Cytoplasmic, Cytosolic Malate Dehydrogenase, Diiodophenylpyruvate Reductase, MDH1, MDHA
    Molekulargewicht
    37.5 kDa
    UniProt
    P40925
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