PLA2G7 Protein (His tag)

Produktnummer ABIN7196844

Produktdetails

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PLA2G7 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human PLA2G7/Lp-PLA2 Protein (His Tag)(Active)

Sequenz: Met 1-Asn 441

Produktmerkmale: A DNA sequence encoding the human PLA2G7 (Q13093-1) precursor (Met 1-Asn 441) was expressed, with a C-terminal polyhistidine tag.

Reinheit: > 88 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2-Thio-PAF), in the presence of 5, 5'Dithiobis(2-nitrobenzoic acid) (DTNB).The specific activity is >5000 pmoles/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM NaAc, 150 mM NaCl, 10 % glycerol, pH 5.0

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Andere Bezeichnung: PLA2G7/Lp-PLA2 (Lp-PLA2 Produkte)

Synonyme: LDL-PLA2 Protein, LP-PLA2 Protein, PAFAD Protein, PAFAH Protein, Lp-PLA2 Protein, PAF-AH Protein, R75400 Protein, zgc:77563 Protein, phospholipase A2 group VII Protein, phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) Protein, PLA2G7 Protein, Pla2g7 Protein, pla2g7 Protein

Hintergrund:

Background: Platelet-activating factor acetylhydrolase; also known as 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycero-phosphocholine esterase; Group-VIIA phospholipase A2; LDL-associated phospholipase A2; PAF 2-acylhydrolase; PLA2G7 and PAFAH; is secreted protein which belongs to the AB hydrolase superfamily and Lipase family. PLA2G7 / PAFAH modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. It has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. PLA2G7 / PAFAH is a potent pro- and anti-inflammatory molecule that has been implicated in multiple inflammatory disease processes; including cardiovascular disease. PLA2G7 also represents an important; potentially functional candidate in the pathophysiology of coronary artery disease (CAD). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PLA2G7 deficiency). It is a trait which is present in 27% of Japanese. It could have a significant physiologic effect in the presence of inflammatory bodily responses.

Synonym: 2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1; EC 3.1.1.47;1-alkyl-2-acetylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); LDL-PLA2; lipoprotein-associated phospholipase A2; LpPLA2; Lp-PLA2; PAF acetylhydrolase; PAF-AH; PAFAHPAF 2-acylhydrolase; phospholipase A2; group VII (platelet-activating factor acetylhydrolase; PLA2G7; plasma); platelet-activating factor acetylhydrolase

Molekulargewicht: 49.2 kDa

Pathways: Peptide Hormone Metabolism