LIFR Protein (His tag)

Produktnummer ABIN7320281

Produktdetails

Target: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses LIFR Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse LIFR/CD118 Protein (His Tag)(Active)

Sequenz: Met 1-Ser 828

Produktmerkmale: A DNA sequence encoding the extracellular domain of mouse LIFR (NP_038612.1) (Met 1-Ser 828) was expressed, with a polyhistidine tag at the C-terminus.

Reinheit: > 95 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to bind mouse LIF-Fc in a functional ELISA.2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 5-20 ng/mL in the presence of 2 ng/mL recombinant human LIF.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Andere Bezeichnung: LIFR/CD118 (LIFR Produkte)

Synonyme: A230075M04Rik Protein, AW061234 Protein, LIF Protein, CD118 Protein, LIF-R Protein, SJS2 Protein, STWS Protein, SWS Protein, leukemia inhibitory factor receptor Protein, LIF receptor alpha Protein, leukemia inhibitory factor receptor alpha Protein, Lifr Protein, LOC397451 Protein, LIFR Protein

Hintergrund:

Background: LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (cardiotrophin like cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1 and cardiotrophin like cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

Synonym: A230075M04Rik,AW061234,LIF

Molekulargewicht: 90 kDa

NCBI Accession: NP_038612

Pathways: JAK-STAT Signalweg, Growth Factor Binding