GZMH Protein (His tag)

Produktnummer ABIN7195990

Produktdetails

Target: GZMH (Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses GZMH Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Granzyme H Protein (His Tag)

Sequenz: Met 1-Leu 246

Produktmerkmale: A DNA sequence encoding the human Granzyme H (NP_219491.1) (Met 1-Leu 246) with a C-terminal polyhistidine tag was expressed.

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: GZMH (Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH))

Andere Bezeichnung: Granzyme H (GZMH Produkte)

Synonyme: GZMH Protein, CCP-X Protein, CGL-2 Protein, CSP-C Protein, CTLA1 Protein, CTSGL2 Protein, granzyme H Protein, LOC617313 Protein, GZMH Protein

Hintergrund:

Background: Granzymes are key components of the immune response that play important roles in eliminating host cells infected by intracellular pathogens. Several granzymes are potent inducers of cell death. A total of eight granzymes (A-G and M) have been identified in the mouse, but only five are known in humans (A, B, H, M and granzyme 3), and granzyme H appears to be specifically human. Human granzyme H is a neutral serine protease that is expressed predominantly in the lymphokine-activated killer (LAK)/natural?killer (NK) compartment of the immune system. In adenovirus-infected cells in which granzyme B (gzmB) and downstream apoptosis pathways are inhibited, granzyme H directly cleaves the adenovirus DNA-binding protein (DBP), a viral component absolutely required for viral DNA replication. This virus demonstrated that gzmH directly induces an important decay in viral DNA replication. Interestingly, gzmH also cleaves the adenovirus 100K assembly protein, a major inhibitor of gzmB, and relieves gzmB inhibition. Granzyme H has a very high amino acid identity (>90%) with many portions of the granzyme B sequence, particularly near the amino terminus of the molecule despite performing a distinct enzymic function.

Synonym: CCP-X;CGL-2;CSP-C;CTLA1;CTSGL2;GZMH

Molekulargewicht: 26.7 kDa

NCBI Accession: NP_219491