DPP4 Protein (Fc Tag)

Produktnummer ABIN7195428

Produktdetails

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses DPP4 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Recombinant Human DPP4/DPPIV/CD26 Protein (Fc Tag)(Active)

Sequenz: Asn 29-Pro 766

Produktmerkmale: A DNA sequence encoding the extracellular domain (Asn 29-Pro 766) of the mature form of human DPPIV (NP_001926.2) was expressed with the fused Fc region of human IgG1 at the N-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to bind recombinant Cynomolgus CXCL12 in a functional ELISA.2. Measured by its ability to bind recombinant Human SDF1b in a functional ELISA.3. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) was 11 nM.4. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) was 32 nM.5. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) was 43 nM.6. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike-His (aa 1-760) was 12 nM.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Andere Bezeichnung: DPP4/DPPIV/CD26 (DPP4 Produkte)

Synonyme: ADABP Protein, ADCP2 Protein, CD26 Protein, DPPIV Protein, TP103 Protein, MGC81966 Protein, MOP9.8 Protein, MOP9_8 Protein, DPP4 Protein, si:ch73-2d23.3 Protein, Cd26 Protein, Dpp-4 Protein, THAM Protein, dipeptidyl peptidase 4 Protein, dipeptidyl-peptidase 4 S homeolog Protein, prolyl oligopeptidase family protein Protein, dipeptidyl-peptidase 4 Protein, dipeptidylpeptidase 4 Protein, DPP4 Protein, dpp4.S Protein, AT5G24260 Protein, dpp4 Protein, Dpp4 Protein

Hintergrund:

Background: Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

Synonym: Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, ADCP-2, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26

Molekulargewicht: 112 kDa

NCBI Accession: NP_001926

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity