DAP Kinase 1 Protein (AA 1-363) (GST tag,His tag)

Produktnummer ABIN7317057

Produktdetails

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-363

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses DAP Kinase 1 Protein ist gelabelt mit GST tag,His tag.

Verwendungszweck: Recombinant Human DAPK1/DAP Kinase 1 Protein (aa 1-363, His & GST Tag)(Active)

Sequenz: Met 1-Leu 363

Produktmerkmale: A DNA sequence encoding the N-terminal segment of human DAPK1 (P53355-1) (Met 1-Leu 363) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Reinheit: > 80 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Andere Bezeichnung: DAPK1/DAP Kinase 1 (DAPK1 Produkte)

Synonyme: DAPK1 Protein, MGC81366 Protein, si:ch211-66i11.1 Protein, wu:fj09c03 Protein, dapk Protein, MGC83745 Protein, 2310039H24Rik Protein, 2810425C21Rik Protein, AI642003 Protein, D13Ucla1 Protein, DAP-Kinase Protein, DAPK Protein, death-associated protein kinase 1 Protein, death associated protein kinase 1 L homeolog Protein, death associated protein kinase 1 Protein, death associated protein kinase 1 S homeolog Protein, LOC427465 Protein, dapk1.L Protein, DAPK1 Protein, dapk1 Protein, dapk1.S Protein, Dapk1 Protein

Hintergrund:

Background: Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains ten ANK repeats, one death domain and one protein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).

Synonym: DAPK

Molekulargewicht: 69.4 kDa

Pathways: MAPK Signalweg, Interferon-gamma Pathway