CD8 alpha Protein (His tag)

Produktnummer ABIN7320295

Produktdetails

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD8 alpha Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse CD8a/Lyt2 Protein (His Tag)(Active)

Sequenz: Met 1-Tyr 196

Produktmerkmale: A DNA sequence encoding the extracellular domain of mouse CD8a (NP_001074579.1) (Met 1-Tyr 196) was expressed, with a C-terminal polyhistidine tag.

Reinheit: > 95 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to bind biotinylated recombinant human B2M in a functional ELISA.2. Measured by its ability to bind biotinylated recombinant human FCGRT+B2M in a functional ELISA.3. Measured by its ability to bind biotinylated recombinant human LCK in a functional ELISA.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8a/Lyt2 (CD8A Produkte)

Synonyme: cd8 Protein, LOC100136450 Protein, BB154331 Protein, Ly-2 Protein, Ly-35 Protein, Ly-B Protein, Lyt-2 Protein, zgc:136643 Protein, CD8 Protein, Leu2 Protein, MAL Protein, p32 Protein, RHACD8-4 Protein, CD8a molecule Protein, uncharacterized LOC100125537 Protein, CD8 alpha Protein, CD8 antigen, alpha chain Protein, T-cell surface glycoprotein CD8 alpha chain Protein, Cd8a Protein, LOC100125537 Protein, LOC100136450 Protein, CD8A Protein, cd8a Protein, LOC100126556 Protein

Hintergrund:

Background: T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I  membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Synonym: BB154331;Ly-2;Ly-35;Ly-B;Lyt-2

Molekulargewicht: 20.3 kDa

NCBI Accession: NP_001074579

Pathways: T-Zell Rezeptor Signalweg