COMP Protein (His tag)

Produktnummer ABIN7317991

Produktdetails

Target: COMP (Cartilage Oligomeric Matrix Protein (COMP))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses COMP Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human COMP Protein (His Tag)(Active)

Sequenz: Met 1-Ala 757

Produktmerkmale: A DNA sequence encoding the human COMP (NP_000086.2) precusor (Met 1-Ala 757) was expressed with a C-terminal polyhistidine tag.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to coated plates (5 μg/ml, 100 μl/well), approximately 40% will adhere specifically after 60 minutes at 37 °C.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: COMP (Cartilage Oligomeric Matrix Protein (COMP))

Andere Bezeichnung: COMP (COMP Produkte)

Synonyme: COMP Protein, EDM1 Protein, EPD1 Protein, MED Protein, PSACH Protein, THBS5 Protein, TSP5 Protein, cartilage oligomeric matrix protein Protein, COMP Protein, sce3551 Protein, CJA_1292 Protein, Comp Protein

Hintergrund:

Background: Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellular matrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressed primarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with high affinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growth plate organization and function. It is suggested to play a role in cell growth and development, and recent studies have revealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor of apoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.

Synonym: EDM1,EPD1,MED,PSACH,THBS5

Molekulargewicht: 82.4 kDa

NCBI Accession: NP_000086