BID Protein (GST tag,His tag)

Produktnummer ABIN7320305

Produktdetails

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses BID Protein ist gelabelt mit GST tag,His tag.

Verwendungszweck: Recombinant Mouse BID Protein (His & GST Tag)(Active)

Sequenz: Met 1-Asp 195

Produktmerkmale: A DNA sequence encoding the mature form of mouse BID (EDK99650.1) (Met 1-Asp 195) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Reinheit: > 95 % as determined by SDS-PAGE

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind  biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.01 ng/mL.3. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind  biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Andere Bezeichnung: BID (BID Produkte)

Synonyme: FP497 Protein, 2700049M22Rik Protein, AI875481 Protein, AU022477 Protein, BID Protein, bid Protein, si:ch211-238n5.6 Protein, fp497 Protein, xbid Protein, DKFZp469E066 Protein, BH3 interacting domain death agonist Protein, BH3 interacting domain death agonist S homeolog Protein, BID Protein, Bid Protein, bida Protein, bid.S Protein, bid Protein

Hintergrund:

Background: The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Synonym: 2700049M22Rik,AI875481,AU022477

Molekulargewicht: 50 kDa

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity