BCL2L1 Protein (His tag)

Produktnummer ABIN7194368

Produktdetails

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses BCL2L1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human BCL2L1/Bcl-XL Protein (His Tag)(Active)

Sequenz: Met 1-Arg 212

Produktmerkmale: A DNA sequence encoding the human BCL2L1 isoform 1 (NP_612815.1) (Met 1-Arg 212) was fused with a polyhistidine tag at the C-terminus.

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.1. Immobilized human BID at 10 μg/mL (100 μl/well) can bind  biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL.2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind  biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.01 ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, pH 8.0

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: BCL2L1/Bcl-XL (BCL2L1 Produkte)

Synonyme: BCL-XL/S Protein, BCL2L Protein, BCLX Protein, BCLXL Protein, BCLXS Protein, Bcl-X Protein, PPP1R52 Protein, bcl-xL Protein, bcl-xS Protein, Bcl(X)L Protein, Bcl-XL Protein, Bcl-Xs Protein, Bcl2l Protein, BclX Protein, bcl-x Protein, Bcl-xl Protein, Bclx Protein, bcl-X Protein, Bcl-xL Protein, BCL-XL Protein, BCL2L1 Protein, bcl-XL Protein, bcl-x(l) Protein, bcl-xl/s Protein, bcl-xs Protein, bcl2l Protein, bclx Protein, bclxl Protein, xR11 Protein, bcl-xl Protein, blp1 Protein, cb1021 Protein, zfBLP1 Protein, BCL2 like 1 Protein, BCL2-like 1 Protein, Bcl2-like 1 Protein, BCL2-like 1 L homeolog Protein, bcl2-like 1 Protein, BCL2L1 Protein, Bcl2l1 Protein, bcl2l1 Protein, bcl2l1.L Protein

Hintergrund:

Background: B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Synonym: Bcl-2-Like Protein 1; Bcl2-L-1; Apoptosis Regulator Bcl-X; Bcl-X;bcl-xL;BCL-XL/S;bcl-xS;BCL2L;BCLX;BCLXL;BCLXS;PPP1R52

Molekulargewicht: 25.2 kDa

NCBI Accession: NP_612815

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling