B-Cell Linker Protein (BLNK) (His tag)
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- Target Alle B-Cell Linker (BLNK) Proteine anzeigen
- B-Cell Linker (BLNK)
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses B-Cell Linker Protein ist gelabelt mit His tag.
- Verwendungszweck
- Recombinant Human BLNK/Ly-57 Protein (His Tag)(Active)
- Sequenz
- Met 1-Ser 456
- Produktmerkmale
- A DNA sequence encoding the human BLNK isoform 1 (AAH18906.1) (Met 1-Ser 456) was fused with a polyhistidine tag at the N-terminus.
- Reinheit
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin-Niveau
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to bind human BTK in a functional ELISA.
- Top Product
- Discover our top product BLNK Protein
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- B-Cell Linker (BLNK)
- Andere Bezeichnung
- BLNK/Ly-57 (BLNK Produkte)
- Synonyme
- BLNK Protein, blnk Protein, MGC147045 Protein, BASH Protein, Bca Protein, Ly-57 Protein, Ly57 Protein, Lyw-57 Protein, SLP-65 Protein, AGM4 Protein, BLNK-S Protein, LY57 Protein, SLP65 Protein, bca Protein, B-cell linker Protein, B cell linker Protein, BLNK Protein, blnk Protein, Blnk Protein
- Hintergrund
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Background: B-cell linker protein, also known as B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65 and BLNK, is a cytoplasm and cell membrane protein which contains one SH2 domain. BLNK is expressed in B-cell lineage and fibroblast cell lines. Highest levels of expression is in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. BLNK functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. BLNK plays a role in the activation of ERK / EPHB2, MAP kinase p38 and JNK. BLNK modulates AP1 activation. It is important for the activation of NF-kappa-B and NFAT. BLNK plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK may be required for the RAC1-JNK pathway. It plays a critical role in orchestrating the pro-B cell to pre-B cell transition. BLNK also plays an important role in BCR-induced B-cell apoptosis. Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) which is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development.
Synonym: B-Cell Linker Protein; B-Cell Adapter Containing a SH2 Domain Protein; B-Cell Adapter Containing a Src Homology 2 Domain Protein; Cytoplasmic Adapter Protein; Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa; SLP-65; BLNK; BASH; SLP65
- Molekulargewicht
- 53 kDa
- Pathways
- BCR Signaling
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