Arylsulfatase A Protein (ARSA) (His tag)

Produktnummer ABIN7194313

Produktdetails

Target: Arylsulfatase A (ARSA)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Arylsulfatase A Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Arylsulfatase A/ARSA Protein (His Tag)(Active)

Sequenz: Met 1-Ala 507

Produktmerkmale: A DNA sequence encoding the human Arylsulfatase A (NP_000478.2) (Met 1-Ala 507) was expressed with a C-terminal polyhistidine tag.

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is >50 pmoles/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM Tris, 0.15 mM NaCl, pH 7.5

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Arylsulfatase A (ARSA)

Andere Bezeichnung: Arylsulfatase A/ARSA (ARSA Produkte)

Synonyme: ARSA Protein, zgc:101575 Protein, arsa Protein, AS-A Protein, ASA Protein, AW212749 Protein, As-2 Protein, As2 Protein, TISP73 Protein, MLD Protein, mld Protein, arylsulfatase A Protein, arylsulfatase Protein, arylsulfatase A, gene 1 S homeolog Protein, ARSA Protein, arsa Protein, arsA Protein, RB6599 Protein, Arsa Protein, arsa.1.S Protein

Hintergrund:

Background: Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH -dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.

Synonym: Arylsulfatase A, ASA, Cerebroside-Sulfatase, ARSA

Molekulargewicht: 53 kDa

NCBI Accession: NP_000478