GRP94 Protein (full length) (rho-1D4 tag)

Produktnummer ABIN7126013

Produktdetails

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Protein-Typ: Recombinant

Proteineigenschaft: full length

Spezies: CHO cells

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses GRP94 Protein ist gelabelt mit rho-1D4 tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)

Sequenz: MRVLWVLGLC CVLLTFGFVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV LKEEASDYLE LDTIKNLVRK YSQFINFPIY VWSSKTETVE EPLEEDEAAK EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH SNRTRLAKLL RFQSSHHSTD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE SEKTKENREA TEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR VKEDEDDKTV LDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID PEAQVEEEPE EEPEDTTEDT EQDEEEEVDA GTEEEEEEEQ ETAKESTAEK DEL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Endoplasmin (Heat shock protein 90 beta family member 1) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Güteklasse: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung: Endoplasmin (Heat shock protein 90 beta family member 1) (HSP90B1 Produkte)

Synonyme: ecgp Protein, gp96 Protein, tra1 Protein, grp94 Protein, DDBDRAFT_0206450 Protein, DDBDRAFT_0215015 Protein, DDB_0206450 Protein, DDB_0215015 Protein, TRA1 Protein, HSP90B1 Protein, ERp99 Protein, GRP94 Protein, TA-3 Protein, Targ2 Protein, Tra-1 Protein, Tra1 Protein, endoplasmin Protein, GP96 Protein, fb61d09 Protein, tra-1 Protein, wu:fb61d09 Protein, wu:fq25g01 Protein, Grp94 Protein, ECGP Protein, Gp96 Protein, ppk98 Protein, hsp108 Protein, heat shock protein 90kDa beta (Grp94), member 1 Protein, heat shock protein Hsp90 family protein Protein, 94 kD glucose-regulated protein Protein, heat shock protein 90 beta family member 1 Protein, heat shock protein 90, beta (Grp94), member 1 Protein, heat shock protein 90, beta (grp94), member 1 Protein, heat shock protein 90kDa beta family member 1 L homeolog Protein, hsp90b1 Protein, grp94 Protein, HSP90B1 Protein, Hsp90b1 Protein, hsp90b1.L Protein

UniProt: G3HQM6

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades