SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (G204R, R203K) (His tag)

Produktnummer ABIN6992418

Produktdetails

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: G204R, R203K

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Nucleocapsid Protein ist gelabelt mit His tag.

Verwendungszweck: SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag

Sequenz: AA 1-419

Produktmerkmale: SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (R203K, G204R). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutations (R203K, G204R) were identified on the SR-linker on the nucleocapsid protein of SARS-CoV-2 variants. Phosphorylation of this SR-link motif in SARS-CoV modulates nucleocapsid multimerization, translational inhibitory activity and cellular localization. The co-occuring mutations R203K and G204R may decrease the overall structural flexibility of SARS-COV-2 N protein.

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Antigendetails

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Andere Bezeichnung: SARS-CoV-2 Nucleocapsid protein (SARS-CoV-2 N Produkte)

Synonyme: nucleocapsid phosphoprotein Protein, N Protein

Substanzklasse: Viral Protein

Hintergrund: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Molekulargewicht: 47.4 kDa