SARS-CoV-2 Spike Protein (D614G, Super Stable Trimer) (His tag)
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- Target Alle SARS-CoV-2 Spike Proteine anzeigen
- SARS-CoV-2 Spike
- Protein-Typ
- Recombinant
- Proteineigenschaft
- D614G, Super Stable Trimer
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Spezies
- SARS Coronavirus-2 (SARS-CoV-2)
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses SARS-CoV-2 Spike Protein ist gelabelt mit His tag.
- Applikation
- ELISA
- Verwendungszweck
- SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)
- Produktmerkmale
- SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and D614G mutation, which has become increasingly common in SARS-CoV-2 viruses from around the world . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
- Reinheit
- > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- PBS
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state
70°C for 3 months under sterile conditions after reconstitution. - Haltbarkeit
- 12 months
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Produkte
- Synonyme
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Substanzklasse
- Viral Protein
- Hintergrund
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molekulargewicht
- 138.0 kDa
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