Recombinant 2019-nCoV envelope Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Val75) of 2019-ncov envelope (Accession #QHD43418.1) fused with a 6xHis,Avi tag at the N-terminus.
Reinheit
> 95 % by SDS-PAGE.
Sterilität
0.22 μm filtered
Endotoxin-Niveau
< 0.1 EU/μg of the protein by LAL method.
Biological Activity Comment
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Envelope at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid with a linear range of 1.2-41.1 ng/mL.
SARS-CoV-2 E
Spezies: SARS Coronavirus-2 (SARS-CoV-2)
Wirt: Escherichia coli (E. coli)
Recombinant
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.5 mg/mL
Buffer
Supplied as a 0.22 μm filtered solution in 20 mM Tris,250 mM NaCl,0.5 % TritonX-100, pH 8.0.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
-80 °C
Informationen zur Lagerung
This product is stable at ≤ -70°C for up to 6 months from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.