Dieses SARS-CoV-2 Envelope Protein ist gelabelt mit His tag,GST tag.
Applikation
SDS-PAGE (SDS)
Verwendungszweck
SARS-CoV-2 (COVID-19) Envelope protein, His Tag
Sequenz
AA 1-75
Produktmerkmale
SARS-CoV-2 Envelope protein, GST,His Tag (ENN-C5128) is expressed from E.coli cells. It contains AA Met 1 - Val 75 (Accession # QHU79206.1). Predicted N-terminus: Met This protein carries a GST tag at the N-terminus and a polyhistidine tag at the C-terminus.
SARS-CoV-2 E
Spezies: SARS Coronavirus-2 (SARS-CoV-2)
Wirt: Escherichia coli (E. coli)
Recombinant
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Buffer
20 mM Tris, 5 mM EDTA, Arginine, pH 8.0
Handhabung
Please avoid repeated freeze-thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Qian, Lei, Patel, Lee, Monaghan-Nichols, Xin, Qiu, Fu: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." in: bioRxiv : the preprint server for biology, (2021) (PubMed).
Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).
The SARS-CoV-2 is composed of a double-layered lipid envelope, including Spike glycoprotein (S), Envelope protein (E), Membrane glycoprotein (M), and Nucleocapsid protein (Nucleocapsid protein, N). Among them, The amino acid sequence of the SARS-CoV-2 envelope protein is 95 % identical of the SARS envelope protein.