SARS-CoV S1 Protein (His tag)

Produktnummer ABIN6952622

Produktdetails

Target: SARS-CoV S1 (SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1))

Protein-Typ: Recombinant

Spezies: SARS Coronavirus (SARS-CoV)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV S1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), ELISA

Verwendungszweck: SARS S1 protein, His Tag (MALS verified)

Sequenz: AA 14-667

Produktmerkmale: SARS S1 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1). Predicted N-terminus: Ser 14
This protein carries a polyhistidine tag at the C-terminus.

Reinheit: >95 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Referenzen

Kondo, Iwatani, Matsuoka, Fujino, Umemoto, Yokomaku, Ishizaki, Kito, Sezaki, Hayashi, Murakami: "Antibody-like proteins that capture and neutralize SARS-CoV-2." in: Science advances, Vol. 6, Issue 42, (2020) (PubMed).

Tosif, Neeland, Sutton, Licciardi, Sarkar, Selva, Do, Donato, Quan Toh, Higgins, Van de Sandt, Lemke, Lee, Shoffner, Flanagan, Arnold, Mordant, Mulholland, Bines, Dohle, Pellicci, Curtis, McNab et al.: "Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. ..." in: Nature communications, Vol. 11, Issue 1, pp. 5703, (2020) (PubMed).

Antigendetails

Target: SARS-CoV S1 (SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1))

Andere Bezeichnung: SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1 Produkte)

Substanzklasse: Viral Protein

Hintergrund: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 74.9 kDa