PCOLCE Protein (AA 26-449) (His tag)

Produktnummer ABIN6388192

Produktdetails

Target: PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))

Protein-Typ: Recombinant

Proteineigenschaft: AA 26-449

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses PCOLCE Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MRDKEETLPL EDGWWGPGTR SAAREDDSIR PFKVETSDEE IHDLHQRIDK FRFTPPLEDS CFHYGFNSNY LKKVISYWRN EFDWKKQVEI LNRYPHFKTK IEGLDIHFIH VKPPQLPAGH TPKPLLMVHG WPGSFYEFYK IIPLLTDPKN HGLSDEHVFE VICPSIPGYG FSEASSKKGF NSVATARIFY KLMLRLGFQE FYIQGGDWGS LICTNMAQLV PSHVKGLHLN MALVLSNFST LTLLLGQRFG RFLGLTERDV ELLYPVKEKV FYSLMRESGY MHIQCTKPDT VGSALNDSPV GLAAYILEKF STWTNTEFRY LEDGGLERKF SLDDLLTNVM LYWTTGTIIS SQRFYKENLG QGWMTQKHER MKVYVPTGFS AFPFELLHTP EKWVRFKYPK LISYSYMVRG GHFAAFEEPE LLAQDIRKFL SVLERQHHHH HH

Reinheit: > 85 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1ug of protein (determined by LAL method)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 50 % glycerol, 1  mM DTT, 0.1M NaCl

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))

Andere Bezeichnung: PCOLCE (PCOLCE Produkte)

Synonyme: PCPE Protein, PCPE-1 Protein, PCPE1 Protein, Astt-2 Protein, Astt2 Protein, P14 Protein, procollagen C-endopeptidase enhancer Protein, procollagen C-endopeptidase enhancer L homeolog Protein, procollagen C-endopeptidase enhancer protein Protein, PCOLCE Protein, Pcolce Protein, pcolce.L Protein

Hintergrund: EPHX1, also known as epoxide hydrolase 1, is an important bioconversion enzyme that converts epoxides from degradation of aromatics into trans-dihydrodiols that can be conjugated and excreted in the body. The epoxide hydrolase acts both in the activation and decryption of the epoxide. This enzyme plays a role in regulating the extensive oxidation state of heterologous and lipid-derived substrates. Mutations in this gene cause preeclampsia, lack of epoxidase, or increased epoxide hydrolase activity. Recombinant human EPHX1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 51.5kDa (442aa) 50-70kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_000111

UniProt: P07099