MAP4K3 Protein (AA 1-321) (His tag)
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- Target Alle MAP4K3 Proteine anzeigen
- MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-321
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Spezies
- E. coli
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses MAP4K3 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL
- Reinheit
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
- Top Product
- Discover our top product MAP4K3 Protein
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Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-894) protein (Strep Tag)
Crystallography grade MAP4K3 Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-894) protein (Strep Tag)Crystallography grade MAP4K3 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-321) protein (His tag)MAP4K3 Spezies: E. coli Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) protein (Myc-DYKDDDDK Tag)MAP4K3 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol.
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))
- Andere Bezeichnung
- glk (MAP4K3 Produkte)
- Synonyme
- GLK Protein, MAPKKKK3 Protein, MEKKK 3 Protein, MEKKK3 Protein, RAB8IPL1 Protein, 4833416M01Rik Protein, 4833416M07Rik Protein, 9530052P13Rik Protein, Glk Protein, mitogen-activated protein kinase kinase kinase kinase 3 Protein, MAP4K3 Protein, Map4k3 Protein
- Hintergrund
- Glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E.coli glk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 37.1kDa (344aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_416889
- UniProt
- P0A6V8
- Pathways
- MAPK Signalweg
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