PRKAB1 Protein (His tag)

Produktnummer ABIN7280002

Produktdetails

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PRKAB1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: 5'-AMP-activated protein kinase subunit beta-1, AMPK, HAMPKb

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Andere Bezeichnung: PRKAB1 (PRKAB1 Produkte)

Synonyme: AMPK Protein, HAMPKb Protein, 1300015D22Rik Protein, AU021155 Protein, E430008F22 Protein, MGC82489 Protein, prkab1 Protein, wu:fk93d05 Protein, wu:fw87e09 Protein, zgc:56652 Protein, zgc:76975 Protein, zgc:92228 Protein, protein kinase AMP-activated non-catalytic subunit beta 1 Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit S homeolog Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit, b Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit, a Protein, PRKAB1 Protein, Prkab1 Protein, prkab1.S Protein, prkab1 Protein, prkab1b Protein, prkab1a Protein

Hintergrund: 5'-AMP-activated protein kinase subunit beta-1, also known as PRKAB1, inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). Recombinant human PRKAB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 32.8 kDa (293aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

NCBI Accession: NP_006244

Pathways: AMPK Signaling, Warburg Effekt