CD80 Protein (CD80) (AA 35-242) (Fc Tag)

Produktnummer ABIN6253222

Produktdetails

Target: CD80

Protein-Typ: Recombinant

Proteineigenschaft: AA 35-242

Spezies: Human

Quelle: CHO Cells

Aufreinigungstag / Konjugat: Dieses CD80 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: CD80 (human):Fc (mouse) (rec.)

Spezifität: The extracellular domain of human CD80 (aa 35-242) is fused to the N-terminus of the Fc region of mouse IgG2a.

Produktmerkmale: Protein. The extracellular domain of human CD80 (aa 35-242) is fused to the N-terminus of the Fc region of mouse IgG2a. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). CD80 (B7-1) and CD86 (B7-2) together with their receptors CD28 and CTLA-4 constitute one of the dominant costimulatory pathways that regulate T cell and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon-gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. It has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

Reinheit: >98 % (SDS-PAGE)

Endotoxin-Niveau: <0.06EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Antigendetails

Target: CD80

Andere Bezeichnung: CD80 (CD80 Produkte)

Synonyme: B7 Protein, B7-1 Protein, B7.1 Protein, BB1 Protein, CD28LG Protein, CD28LG1 Protein, LAB7 Protein, B71 Protein, Cd28l Protein, Ly-53 Protein, Ly53 Protein, MIC17 Protein, TSA1 Protein, CD80 molecule Protein, Cd80 molecule Protein, CD80 antigen Protein, CD80 Protein, Cd80 Protein

Hintergrund:

Alternate Names/Synonyms: B7-1

Product Description: CD80 (B7-1) and CD86 (B7-2) together with their receptors CD28 and CTLA-4 constitute one of the dominant costimulatory pathways that regulate T cell and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon-gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. It has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

NCBI Accession: NP_005182

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints