Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Biologische Aktivität
Active
Proteineigenschaft
AA 30-252
Spezies
Ratte
Quelle
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses Tissue factor Protein ist gelabelt mit His tag.
Applikation
Cell Culture (CC), Activity Assay (AcA)
Produktmerkmale
Tag location: N-terminal His Tag
Reinheit
> 95 %
Biological Activity Comment
TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of TF with EGF.
Crystallography grade
F3
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Applikationshinweise
Isoelectric Point: 9.2
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Buffer
20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
Konservierungsmittel
Dithiothreitol (DTT), Other preservative, ProClin
Vorsichtsmaßnahmen
This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Target
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))