Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Protein-Typ
Recombinant
Proteineigenschaft
AA 33-251
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses Tissue factor Protein ist gelabelt mit His tag.
Verwendungszweck
Human Coagulation Factor III/Tissue Factor Protein
Sequenz
Ser33-Glu251
Produktmerkmale
Recombinant Human Coagulation Factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Glu251.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Coagulation Factor III, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
F3
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Crystallography grade
F3
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
Molekulargewicht
25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.