LCAT Protein (AA 41-210) (His tag)

Produktnummer ABIN6239826

Produktdetails

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Biologische Aktivität: Active

Proteineigenschaft: AA 41-210

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses LCAT Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 90 %

Biological Activity Comment: LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of LCAT with APOA1.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 6.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Andere Bezeichnung: Lecithin Cholesterol Acyltransferase (LCAT) (LCAT Produkte)

Synonyme: AI046659 Protein, D8Wsu61e Protein, MGC82035 Protein, lcat Protein, MGC88964 Protein, LCAT Protein, lecithin-cholesterol acyltransferase Protein, lecithin cholesterol acyltransferase Protein, lecithin-cholesterol acyltransferase L homeolog Protein, solute carrier family 12 member 4 Protein, fragile site, aphidicolin type, common, fra(13)(q13.2) Protein, LCAT Protein, Lcat Protein, lcat.L Protein, lcat Protein, SLC12A4 Protein, FRA13A Protein

Hintergrund: Alternative Names: phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase

Molekulargewicht: 22kDa

UniProt: P16301

Pathways: Lipid Metabolism